EVALUATION OF BENEFITS FROM OZONE THERAPY IN PATIENTS WITH CHRONIC PERIODONTITIS
Keywords:
Ozonated olive oil, BANA test, conservative periodontal treatmentAbstract
Aim: The study evaluated the effects of subgingival application of ozonated olive oil as adjuvant therapy to classic non-surgical conservative periodontal treatment.
Material and methods: Sixty patients with moderate and advanced periodontal disease were divided into two groups: group 1, in which only conservative periodontal treatment (scaling and root planning) was applied, and group 2, in which, in addition to the conservative treatment, ozonated olive oil (Ozone Gold-Germany, GmbH, 80 mg) was also applied. The subgingival application of ozonated olive oil was performed right after the conservative treatment and also, on days 7, 14 and 21. Clinical parameters included DPI, IGI, GBI, CAL. BANA test was performed to detect the presence of the three anaerobic periopathogenic bacterial species (T. denticola, T. forsythia, P. gingivalis) in subgingival dental plaque. Clinical parameters and BANA test were recorded at the beginning, before starting conservative treatment and 1 month afterwards.
Results: The results indicated an improvement in all clinical parameters in both groups, with a statistical significance in the group in which ozonated olive oil was applied in addition to conservative periodontal treatment. The combined treatment showed a significantly greater success than the conservative one in terms of the presence of the three anaerobic periopathogenic bacterial species (T. denticola, T. forsythia, P. gingivalis).
Conclusion: Topical application of ozonated olive oil in addition to conservative periodontal treatment may serve as a potential atraumatic, promising antimicrobial agent in the non-surgical treatment of periodontal disease.
References
Locker D, Slade GD, Murray H. Epidemiology of periodontal disease among older adults: A review. Periodontol 2000 1998; 16: 16-33. doi: 10.1111/j.1600-0757.1998.tb00113.x.
Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. Lancet 2005; 366(9499): 1809-1820. doi: 10.1016/S0140-6736(05)67728-8.
Savage A, Eaton KA, Moles DR, Needleman I. A systematic review of definitions of periodontitis and methods that have been used to identify this disease. J Clin Periodontol 2009; 36(6): 458-467. doi: 10.1111/j.1600-051X.2009.01408.x.
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998; 25(2): 134-144. doi: 10.1111/j.1600-051x.1998.tb02419.x.
Loesche WJ, Bretz WA, Kerschensteiner D, Stoll J, Socransky SS, Hujoel P, et al. Development of a diagnostic test for anaerobic periodontal infections based on plaque hydrolysis of benzoyl-DL-arginine-naphthylamide. J Clin Microbiol 1990; 28(7): 1551-1559. doi: 10.1128/jcm.28.7.1551-1559.1990.
Nambiar S, Malothu S, Karmakar S, Varkey A, Chandra D, Chava VK. Comparison of Ozonated Olive Oil and Chlorhexidine Gel as an Adjunct to Nonsurgical Periodontal Therapy for the Treatment of Chronic Periodontitis: A Randomized Controlled Clinical Trial. J Pharm Bioallied Sci 2022; 14(Suppl 1): S94-S98. doi: 10.4103/jpbs.jpbs_565_21.
Deepthi R, Bilichodmath S. Ozone Therapy in Periodontics: A Meta-analysis. Contemp Clin Dent 2020; 11(2): 108-115. doi: 10.4103/ccd.ccd_79_19.
Saini R. Ozone therapy in dentistry: A strategic review. J Nat Sci Biol Med 2011; 2(2): 151-153. doi: 10.4103/0976-9668.92318.
Silness J, Loe H. Periodontal disease in pregnancy. 2. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 1964; 22: 121-135. doi: 10.3109/00016356408993968.
Loe H, Silness J. Periodontal disease in pregnancy I. Prevalence and severity. Acta Odontol Scand 1963; 21: 533-551. doi: 10.3109/00016356309011240.
Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J 1975; 25(4): 229-235. PMID: 1058834.
Highfield, J. Diagnosis and classification of periodontal disease. Australian Dental Journal 2009; 54(Suppl 1): S11-S26. doi: 10.1111/j.1834-7819.2009.01140.x.
Graetz C, Ehrenthal JC, Kowalski R, Cyris M, Geiken A, Dörfer CE. Periodontal maintenance: individual patient responses and discontinuations. BMC Oral Health 2022; 22(1): 610. doi: 10.1186/s12903-022-02655-8.
Ramirez-Peña AM, Sánchez-Pérez A, Campos-Aranda M, Hidalgo-Tallón FJ. Ozone in Patients with Periodontitis: A Clinical and Microbiological Study. J Clin Med 2022; 11(10): 2946. doi: 10.3390/jcm11102946.
Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 2004; 30(11): 778-781. doi: 10.1097/00004770-200411000-00007.
Ramzy MI, Gomaa HE, Mostafa MI, Zaki BM. Management of aggressive periodontitis using ozonized water. Egypt Med JNR C 2005; 6: 229-245.
Huth KC, Quirling M, Lenzke S, Paschos E, Kamereck K, Brand K, et al. Effectiveness of ozone against periodontal pathogenic microorganisms. Eur J Oral Sci 2011; 119(3): 204-210. doi: 10.1111/j.1600-0722.2011.00825.x.
Staehelin J, Hoigne J. Decomposition of ozone in water in the presence of organic solutes acting as promoters and inhibitors of radical chain reactions. Environ Sci Technol 1985; 19(12): 1206-1213. doi: 10.1021/es00142a012.
Hoigne J, Bader H. The role of hydroxyl radical reactions in ozonation processes in aqueous solutions. Water Res 1976; 10(5): 377-386. doi: 10.1016/0043-1354(76)90055-5.
Shin WT, Mirmiran A, Yiacoumi S, Tsouris C. Ozonation using micro bubbles formed by electric fields. Separat Purif Technol 1999; 15(3): 271-282. doi: 10.1016/S1383-5866(98)00107-5.
Gupta G, Mansi B. Ozone therapy in periodontics. J Med Life 2012; 5(1): 59-67. PMID: 22574088.
Sorokina S, Lukinych L. Ozone therapy as a part of a complex treatment of a paradontium disease. The medical academy of Nizhni Novgorod, RussiaSource: 2nd International Symposium on Ozone Applications, Havana, Cuba 1997; pp. 24-26.
Piva A, Avantaggiato P, Candotto V, Pellati A, Moreo G. The use of ozone therapy for treatment of periodontal disease: a split-mouth, randomized, controlled clinical trial. J Biol Regul Homeost Agents 2020; 34(3 Suppl 1): 91-98. PMID: 32618165.
Patel PV, Patel A, Kumar S, Holmes JC. Effect of subgingival application of topical ozonated olive oil in the treatment of chronic periodontitis: a randomized, controlled, double blind, clinical and microbiological study. Minerva Stomatol 2012; 61(9): 381-398. PMID: 22976566.
Colombo M, Gallo S, Garofoli A, Poggio C, Arciola CR, Scribante A. Ozone Gel in Chronic Periodontal Disease: A Randomized Clinical Trial on the Anti-Inflammatory Effects of Ozone Application. Biology 2021; 10(7): 625. doi: 10.3390/biology10070625.
Jain N, Jain GK, Javed S, Iqbal Z, Talegaonkar S, Ahmad FJ, et al. Recent approaches for treatment of periodontitis. Drug Discov Today 2008; 13(21-22): 932-943. doi: 10.1016/j.drudis.2008.07.010.
Rosentein ED, Kushner LJ, Kramer N, Kazandjian G. Pilot study of dietary fatty acid supplementation in the treatment of adult periodontitis. Prostaglandins Leukot Essent Fatty Acids 2003; 68(3): 213e8. doi: 10.1016/S0952-3278(02)00272-7.
Shoukheba MYM, Ali ShA. The effects of subgingival application of ozonated olive oil gel in patient with localized aggressive periodontitis. A clinical and bacteriological study. Tanta Dental Journal. 2014; 11(1): 63-73. doi: 10.1016/j.tdj.2014.04.001.
Eick S, Tigan M, Sculean A. Effect of ozone on periodontopathogenic species - an in vitro study. Clin Oral Invest 2012; 16(2): 537-544. doi: 10.1007/s00784-011-0515-1.
