• Ljubica Tasheva Institute of Medical Histology and Embryology, Faculty of Medicine, Ss. Cyril and Methodius University, Skopje, Republic of North Macedonia
  • Irena Petrova-Kostadinova Institute of Medical Histology and Embryology, Faculty of Medicine, Ss. Cyril and Methodius University, Skopje, Republic of North Macedonia
  • Natasha Stojkovska Institute of Medical Histology and Embryology, Faculty of Medicine, Ss. Cyril and Methodius University, Skopje, Republic of North Macedonia
  • Elida Mitevska Institute of Medical Histology and Embryology, Faculty of Medicine, Ss. Cyril and Methodius University, Skopje, Republic of North Macedonia
  • Lena Mazhenkovska-Kakasheva Institute of Medical Histology and Embryology, Faculty of Medicine, Ss. Cyril and Methodius University, Skopje, Republic of North Macedonia


squamous cell carcinoma, CD34, neovascularization, histological differentiation


Introduction: Abnormal angiogenesis is described in tumor growth and it facilitates its metastatic spread. Tumors with high angiogenic activity belong to the category of aggressive tumors with a poor prognosis for patients.

The aim of this study was to determine neovascularization at the invasive front of the tumor stroma in skin squamous cell carcinoma (SCC) in relation to the healthy skin and the ratio of blood vessel density in the skin SCC with a different depth of invasion and different degree of histological differentiation.

Materials and methods: The material consisted of surgical specimens obtained from 30 patients with skin SCC, who underwent surgery at the University Clinic for Plastic and Reconstructive Surgery and University Clinic for Maxillofacial Surgery. Samples were analyzed by standard paraffin technique stained by hematoxylin-eosin and immunohistochemically with antibodies against smooth muscle actin (SMA) and CD34.

Results: The difference found in the neovascularization density in neoplasms with different degree of differentiation (G1, G2, G3) was statistically significant. The differences in the depth of stromal invasion in skin SCC registered in neoplasms with different degree of histological differentiation (G) showed a statistical significance for G1, G2, G3. The density of neovascularization in skin SCC was in a positive correlation with the depth of invasion.

Conclusion: The increased vascularization at the invasive front of a neoplasm in SCC with deeper invasion and higher grade has pointed out to its possible role in neoplasm progression.


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